DIO3 antibody (C-Term)

Catalog No. ABIN5532317

This Rabbit Polyclonal antibody specifically detects DIO3 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for DIO3 antibody (C-Term) (ABIN5532317)

Target: DIO3 (Deiodinase, Iodothyronine, Type III (DIO3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DIO3 Antibody

Binding Specificity: AA 250-278, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DIO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DIO3

Target: DIO3 (Deiodinase, Iodothyronine, Type III (DIO3))

Alternative Name: DIO3

Background: DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

Molecular Weight: 34 kDa

Gene ID: 1735

UniProt: P55073

Pathways: Hormone Activity