DIO3 antibody (C-Term)

Catalog No. ABIN951914

Product Details anti-DIO3 Antibody

Target: DIO3 (Deiodinase, Iodothyronine, Type III (DIO3))

Binding Specificity: AA 249-278, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes Human DIO3 (C-term).

Purification: Affinity Chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 249~278 amino acids from the C-terminal region of human DIO3

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DIO3

Target: DIO3 (Deiodinase, Iodothyronine, Type III (DIO3))

Alternative Name: DIO3 (DIO3 Products)

Synonyms: 5DIII antibody, DIOIII antibody, txdi3 antibody, dioiii antibody, XL-15 antibody, itdi3 antibody, D3 antibody, TXDI3 antibody, iodothyronine deiodinase 3 antibody, deiodinase, iodothyronine, type 3 antibody, deiodinase, iodothyronine, type III antibody, deiodinase, iodothyronine type III antibody, deiodinase, iodothyronine, type 3 L homeolog antibody, DIO3 antibody, dio3 antibody, Dio3 antibody, dio3.L antibody

Background: DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.Synonyms: 5DIII, DIOIII, ITDI3, TXDI3, Type 3 DI, Type III iodothyronine deiodinase, Type-III 5'-deiodinase

Molecular Weight: 31451 Da

Gene ID: 1735

NCBI Accession: NP_001353

Pathways: Hormone Activity