ADD3 antibody (C-Term)

Catalog No. ABIN5532513

The Rabbit Polyclonal anti-ADD3 antibody has been validated for WB. It is suitable to detect ADD3 in samples from Human.

Quick Overview for ADD3 antibody (C-Term) (ABIN5532513)

Target: ADD3 (Adducin 3 (Gamma) (ADD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADD3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ADD3 Antibody

Binding Specificity: AA 552-580, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ADD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 552-580 amino acids from the C-terminal region of human ADD3.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ADD3

Target: ADD3 (Adducin 3 (Gamma) (ADD3))

Alternative Name: ADD3

Background: Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.

Molecular Weight: 79 kDa

Gene ID: 120

UniProt: Q9UEY8