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Actin antibody (AA 189-218)

This anti-Actin antibody is a Rabbit Polyclonal antibody detecting Actin in WB and IHC (p). Suitable for Human.
Catalog No. ABIN5532808

Quick Overview for Actin antibody (AA 189-218) (ABIN5532808)

Target

See all Actin Antibodies
Actin

Reactivity

  • 101
  • 78
  • 74
  • 27
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Human

Host

  • 87
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Rabbit

Clonality

  • 79
  • 56
Polyclonal

Conjugate

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This Actin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

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    AA 189-218

    Purification

    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

    Immunogen

    This Actin (ACTB/ACTC) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the Central region of human Actin (ACTB/ACTC).

    Isotype

    Ig Fraction
  • Application Notes

    For WB starting dilution is: 1:1000

    For IHC-P starting dilution is: 1:10~50

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.8 mg/mL

    Buffer

    Supplied in PBS with 0.09 % (W/V) sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target

    Actin

    Background

    Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

    Molecular Weight

    42 kDa

    Gene ID

    70

    UniProt

    P68032
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