Deoxyuridine Triphosphatase (DUT) (AA 170-198), (C-Term) antibody

Catalog No. ABIN5534888

This Rabbit Polyclonal antibody specifically detects in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Deoxyuridine Triphosphatase (DUT) (AA 170-198), (C-Term) antibody (ABIN5534888)

Target: Deoxyuridine Triphosphatase (DUT)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 170-198, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DUT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the C-terminal region of human DUT.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details

Target: Deoxyuridine Triphosphatase (DUT)

Alternative Name: DUT

Background: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Molecular Weight: 27 kDa

Gene ID: 1854

UniProt: P33316