ERO1L antibody (AA 105-118)

Catalog No. ABIN5539459

This anti-ERO1L antibody is a Goat Polyclonal antibody detecting ERO1L in WB, ELISA and IHC. Suitable for Human.

Quick Overview for ERO1L antibody (AA 105-118) (ABIN5539459)

Target: ERO1L (ERO1-Like Protein alpha (ERO1L))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ERO1L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ERO1L Antibody

Binding Specificity: AA 105-118

Purpose: ERO1-like (aa105-118)

Sequence: QSDEVPDGIK SASY

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Approx 70 kDa band observed in lysates of cell lines A431, HeLa, Jurkat (calculated MW of 54.4 kDa according to NP_055399.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3 μg/mL. Primary

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ERO1L

Target: ERO1L (ERO1-Like Protein alpha (ERO1L))

Alternative Name: ERO1L

Background: ERO1L, ERO1-like (S. cerevisiae), ERO1-alpha, ERO1-L, ERO1-L-alpha, ERO1-like protein alpha, endoplasmic oxidoreductin-1-like protein, oxidoreductin-1-L-alpha

Gene ID: 30001

NCBI Accession: NP_055399

Pathways: Peptide Hormone Metabolism, ER-Nucleus Signaling, Brown Fat Cell Differentiation