Iba1 antibody (C-Term) (Biotin)

Catalog No. ABIN5539468

This anti-Iba1 antibody is a Goat Polyclonal antibody detecting Iba1 in WB and ELISA. Suitable for Human and Rat.

Quick Overview for Iba1 antibody (C-Term) (Biotin) (ABIN5539468)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Iba1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Iba1 Antibody (Biotin)

Binding Specificity: C-Term

Purpose: AIF1 / IBA1 (isoform 1 and 3), biotinylated

Sequence: TGPPAKKAIS ELP.

Specificity: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 17 kDa band observed in Rat Brain lysates (calculated MW of 16.7 kDa according to NP_001614.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: AIF1, allograft inflammatory factor 1, AIF-1, IBA1, IRT-1, IRT1, allograft inflammatory factor-1 splice variant Hara-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, protein G1

Gene ID: 199, 11629, 29427

NCBI Accession: NP_116573, NP_004838, NP_001614

Pathways: Smooth Muscle Cell Migration