Deoxyuridine Triphosphatase (DUT) (Internal Region) antibody

Catalog No. ABIN5539501

This anti- antibody is a Goat Polyclonal antibody detecting in WB, IHC and ELISA. Suitable for Human.

Quick Overview for Deoxyuridine Triphosphatase (DUT) (Internal Region) antibody (ABIN5539501)

Target: Deoxyuridine Triphosphatase (DUT)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details

Binding Specificity: Internal Region

Purpose: dUTPase / DUT

Sequence: QLRFARLSEH ATAPT

Specificity: This antibody is expected to recognize all reported isoforms (NP_001020419.1, NP_001939.1, NP_001020420.1).

Cross-Reactivity: Cow, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis and Tonsil. Recommended concentration: 5 μg/mL.

Western Blot: Approx 20 kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 17.7 kDa according to NP_001939.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details

Target: Deoxyuridine Triphosphatase (DUT)

Alternative Name: DUT

Background: DUT, deoxyuridine triphosphatase, dUTPase, dUTP nucleotidohydrolase, dUTP pyrophosphatase, deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Gene ID: 1854

NCBI Accession: NP_001020419, NP_001939, NP_001020420