S100A8 antibody (Internal Region)

Catalog No. ABIN5539505

This Goat Polyclonal antibody specifically detects S100A8 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for S100A8 antibody (Internal Region) (ABIN5539505)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-S100A8 Antibody

Binding Specificity: Internal Region

Purpose: S100A8

Sequence: KGADVWFKEL DINT

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 11 kDa band observed in Human Lung lysates (calculated MW of 10.8 kDa according to NP_002955.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8

Background: S100A8, S100 calcium binding protein A8, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8, MRP-8, OTTHUMP00000015329, S100 calcium-binding protein A8 (calgranulin A), calgranulin A, calgranulin-A, calprotectin L1L subunit, cystic fibrosis

Gene ID: 6279

NCBI Accession: NP_002955

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins