TNNT1 antibody (Internal Region)

Catalog No. ABIN5539506

The Goat Polyclonal anti-TNNT1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect TNNT1 in samples from Human.

Quick Overview for TNNT1 antibody (Internal Region) (ABIN5539506)

Target: TNNT1 (Slow Skeletal Troponin T (TNNT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TNNT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-TNNT1 Antibody

Binding Specificity: Internal Region

Purpose: TNNT1

Sequence: EKAQELSDWI HQLE

Specificity: This antibody is expected to recognize all reported isoforms (NP_003274.3, NP_001119604.1, NP_001119605.1).

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5 μg/mL.

Western Blot: Approx 37+35 kDa bands observed in Human Skeletal Muscle lysates A single 37 kDa band was observed in some lysates.(calculated MW of 32.9 kDa according to NP_003274.3). This molecular weight is routinely observed by other sources. Recommende

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TNNT1

Target: TNNT1 (Slow Skeletal Troponin T (TNNT1))

Alternative Name: TNNT1

Background: TNNT1, troponin T type 1 (skeletal, slow), ANM, FLJ98147, MGC104241, STNT, TNT, TNTS, slow skeletal muscle troponin T, troponin T, slow skeletal muscle, troponin-T1, skeletal, slow

Gene ID: 7138

NCBI Accession: NP_003274, NP_001119604, NP_001119605