LDHB antibody (Internal Region)

Catalog No. ABIN5539548

The Goat Polyclonal anti-LDHB antibody has been validated for WB and ELISA. It is suitable to detect LDHB in samples from Human, Rat, Mouse and Pig.

Quick Overview for LDHB antibody (Internal Region) (ABIN5539548)

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Reactivity: Human, Rat, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This LDHB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-LDHB Antibody

Binding Specificity: Internal Region

Purpose: LDHB

Sequence: NARGLTSVIN QKLK

Specificity: Reported variants represent identical protein: NP_002291.1, NP_001167568.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 36.6 kDa according to NP_002291.1). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: LDHB

Background: LDHB, lactate dehydrogenase B, LDH-B, LDH-H, LDHBD, TRG-5, L-lactate dehydrogenase B chain, LDH heart subunit, lactate dehydrogenase H chain, renal carcinoma antigen NY-REN-46

Gene ID: 3945, 16832, 24534

NCBI Accession: NP_002291

Pathways: Warburg Effect