LDHB antibody (Internal Region)

Catalog No. ABIN5539548

Product Details anti-LDHB Antibody

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This LDHB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: LDHB

Sequence: NARGLTSVIN QKLK

Specificity: Reported variants represent identical protein: NP_002291.1, NP_001167568.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 36.6 kDa according to NP_002291.1). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: LDHB (LDHB Products)

Synonyms: LDH-B antibody, LDH-H antibody, LDHBD antibody, TRG-5 antibody, AI790582 antibody, H-Ldh antibody, Ldh-2 antibody, Ldh2 antibody, ldh-h antibody, ldh2 antibody, ldhb antibody, ldhba antibody, trg-5 antibody, LDHB antibody, LDH-B-A antibody, wu:fa16d07 antibody, wu:fa20c10 antibody, wu:fa96a08 antibody, LDH-B-B antibody, zgc:92882 antibody, lactate dehydrogenase B antibody, lactate dehydrogenase B S homeolog antibody, lactate dehydrogenase Ba antibody, lactate dehydrogenase Bb antibody, LDHB antibody, Ldhb antibody, ldhb.S antibody, ldhb antibody, ldhba antibody, ldhbb antibody

Background: LDHB, lactate dehydrogenase B, LDH-B, LDH-H, LDHBD, TRG-5, L-lactate dehydrogenase B chain, LDH heart subunit, lactate dehydrogenase H chain, renal carcinoma antigen NY-REN-46

Gene ID: 3945, 16832, 24534

NCBI Accession: NP_002291

Pathways: Warburg Effect