LIF antibody (AA 28-39)

Catalog No. ABIN5539549

This anti-LIF antibody is a Goat Polyclonal antibody detecting LIF in WB and ELISA. Suitable for Mouse and Rat.

Quick Overview for LIF antibody (AA 28-39) (ABIN5539549)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This LIF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-LIF Antibody

Binding Specificity: AA 28-39

Purpose: LIF (aa28-39)

Sequence: TPVNATCAIR HP

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 23 kDa band observed in Mouse and Rat Colon lysates (calculated MW of 22.3 kDa according to Mouse NP_032527.1 and 22.1 kDa according to Rat NP_071532.2). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation 1 hour at room tempera

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: LIF, leukemia inhibitory factor (cholinergic differentiation factor), CDF, DIA, HILDA, D factor, MLPLI, OTTHUMP00000198698, cholinergic differentiation factor, differentiation inhibitory activity, differentiation stimulating factor, differentiation-induci

Gene ID: 3976, 16878, 60584

NCBI Accession: NP_002300

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding