PKC mu antibody (AA 233-246)

Catalog No. ABIN5539575

The Goat Polyclonal anti-PKC mu antibody has been validated for WB, ELISA, IHC and IP. It is suitable to detect PKC mu in samples from Human.

Quick Overview for PKC mu antibody (AA 233-246) (ABIN5539575)

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PKC mu antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Grade: Verified

Product Details anti-PKC mu Antibody

Binding Specificity: AA 233-246

Purpose: PRKD1 (aa233-246)

Sequence: QKSPSESFIG REKR

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: This antibody has been confirmed in human pancreatic adenocarcinoma (PDA) according to Döppler et al, Sci Rep. 2016 Sep 21,6:33758 PMID 27649783.

Western Blot: In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100 kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunoprecipitation: PKD1 was immunoprecipitated from Hek293T cells (as above). Immunoprecipitates were analyzed by Western Blot probed with the same antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PKC mu

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Alternative Name: PRKD1

Background: PRKD1, PKC-MU, PKCM, PKD, PRKCM,

Gene ID: 5587, 18760, 85421

NCBI Accession: NP_002733

Pathways: Myometrial Relaxation and Contraction