VEGFA antibody (AA 288-299)

Catalog No. ABIN5539606

This anti-VEGFA antibody is a Goat Polyclonal antibody detecting VEGFA in WB, IHC and ELISA. Suitable for Human.

Quick Overview for VEGFA antibody (AA 288-299) (ABIN5539606)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-VEGFA Antibody

Binding Specificity: AA 288-299

Purpose: VEGFA (aa288-299)

Sequence: RIKPHQGQHI GE

Specificity: This antibody is expected to recognize all reported isoforms.

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of cell line HEK293 (calculated MW of 45.5 kDa according to NP_001020537.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Testing has shown that this antibody stains mitochondria in Mouse NIH3T3 cells at 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA

Background: VEGFA, vascular endothelial growth factor A, MGC70609, MVCD1, VEGF, VPF, OTTHUMP00000016487, OTTHUMP00000016488, OTTHUMP00000165985, OTTHUMP00000165986, OTTHUMP00000165987, OTTHUMP00000224107, OTTHUMP00000224108, OTTHUMP00000224109, OTTHUMP00000224153, OT

Gene ID: 7422

NCBI Accession: NP_001020537, NP_003367, NP_001020538, NP_001020539

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling