IRS4 antibody (AA 633-647)

Catalog No. ABIN5539610

This Goat Polyclonal antibody specifically detects IRS4 in WB, ELISA and IF. It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for IRS4 antibody (AA 633-647) (ABIN5539610)

Target: IRS4 (Insulin Receptor Substrate 4 (IRS4))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IRS4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-IRS4 Antibody

Binding Specificity: AA 633-647

Purpose: Irs4 (mouse aa633-647)

Sequence: DRGTKERKEA KEVRD

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in Mouse Heart lysates (calculated MW of 130 kDa according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 20

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: This product has been successfully used in IF on Mouse: Harriet M Jackson et al, BMC Genomics. 2013 Nov 25,14:831. PMID: 24274089.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-IRS4 antibody (ABIN5539610)

Ikink, Boer, Bakker, Hilkens: "IRS4 induces mammary tumorigenesis and confers resistance to HER2-targeted therapy through constitutive PI3K/AKT-pathway hyperactivation." in: Nature communications, Vol. 7, pp. 13567, (2018) (PubMed).

Target Details for IRS4

Target: IRS4 (Insulin Receptor Substrate 4 (IRS4))

Alternative Name: Irs4

Background: Irs4, insulin receptor substrate 4, IRS-4, RP23-415G1.3, phosphoprotein of 160 kDa, pp160

Gene ID: 16370, 315350

NCBI Accession: NP_034702