ARPC1A antibody (C-Term)

Catalog No. ABIN5539619

This Goat Polyclonal antibody specifically detects ARPC1A in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ARPC1A antibody (C-Term) (ABIN5539619)

Target: ARPC1A (Actin Related Protein 2/3 Complex, Subunit 1A, 41kDa (ARPC1A))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ARPC1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ARPC1A Antibody

Binding Specificity: C-Term

Purpose: ARPC1A

Sequence: DFKTLESSIQ GLR

Specificity: This antibody is expected to recognize both reported isoforms (NP_006400.2, NP_001177925.1). No cross-reactiviry expected with ARPC1B.

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of cell line HeLa (calculated MW of 41.6 kDa according to NP_006400.2). Approx 37 kDa band observed in fetal Mouse Brain and adult Rat Brain lysates (calculated MW of 41.6 kDa according to Mouse NP_062741.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ARPC1A

Target: ARPC1A (Actin Related Protein 2/3 Complex, Subunit 1A, 41kDa (ARPC1A))

Alternative Name: ARPC1A

Background: ARPC1A, actin related protein 2/3 complex, subunit 1A, 41 kDa, Arc40, SOP2Hs, SOP2L, OTTHUMP00000206034, SOP2-like protein, actin binding protein (Schizosaccharomyces pombe sop2-like), actin-related protein 2/3 complex subunit 1A

Gene ID: 10552, 56443, 81824

NCBI Accession: NP_006400, NP_001177925

Pathways: Regulation of Actin Filament Polymerization