UCP2 antibody (Internal Region)

Catalog No. ABIN5539644

The Goat Polyclonal anti-UCP2 antibody has been validated for WB, ELISA and IF. It is suitable to detect UCP2 in samples from Human, Mouse and Rat.

Quick Overview for UCP2 antibody (Internal Region) (ABIN5539644)

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This UCP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-UCP2 Antibody

Binding Specificity: Internal Region

Purpose: Uncoupling protein 2 / UCP2

Sequence: DSVKQFYTKG SEH

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Weak positive staining of red and white pulp cells in parafin embedded Human Kidney, at 6-7 μg/mL.

Western Blot: Approx 37 kDa band observed in Rat Adipose and 28-30 kDa in Mouse Spleen lysates (calculated MW of 33.4 kDa according to Mouse NP_035801.3 and 33.4 kDa according to Rat NP_062227.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Mitochondria of MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UCP2

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Alternative Name: UCP2

Background: UCP2, uncoupling protein 2 (mitochondrial, proton carrier), SLC25A8, UCPH , uncoupling protein 2, uncoupling protein-2

Gene ID: 7351, 22228, 54315

NCBI Accession: NP_003346

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Proton Transport