Cytokeratin 19 antibody (AA 285-298) (Biotin)

Catalog No. ABIN5539646

This Goat Polyclonal antibody specifically detects Cytokeratin 19 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Cytokeratin 19 antibody (AA 285-298) (Biotin) (ABIN5539646)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Cytokeratin 19 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Cytokeratin 19 Antibody (Biotin)

Binding Specificity: AA 285-298

Purpose: cytokeratin 19 (aa285-298), Biotinylated

Sequence: HTEQLQMSRS EVTD.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in Human Colorectal cancer lysates (calculated MW of 44.1 kDa according to NP_002267.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: KRT19

Background: KRT19, keratin 19, type I, CK19, K19, K1CS, 40- kDa keratin intermediate filament, CK-19, cytokeratin 19, cytokeratin-19, keratin, type I, 40-kd, keratin-19

Gene ID: 3880

NCBI Accession: NP_002267