CBX3 antibody (Internal Region) (Biotin)

Catalog No. ABIN5539652

This Goat Polyclonal antibody specifically detects CBX3 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for CBX3 antibody (Internal Region) (Biotin) (ABIN5539652)

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CBX3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CBX3 Antibody (Biotin)

Binding Specificity: Internal Region

Purpose: CBX3 / HP1 Gamma, Biotinylated

Sequence: CEAFLNSQKA GKEKD.

Specificity: No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein: NP_009207.2, NP_057671.2

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 20 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 20.8 kDa according to Mouse NP_031650.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CBX3

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Alternative Name: CBX3

Background: CBX3, chromobox 3, HECH, HP1-GAMMA, HP1Hs-gamma, HP1 gamma homolog, chromobox homolog 3 (HP1 gamma homolog, Drosophila), heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, modifier 2 protein

Gene ID: 11335, 12417, 297093

NCBI Accession: NP_009207