CA12 antibody (AA 188-199) (Biotin)

Catalog No. ABIN5539693

This anti-CA12 antibody is a Goat Polyclonal antibody detecting CA12 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for CA12 antibody (AA 188-199) (Biotin) (ABIN5539693)

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CA12 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CA12 Antibody (Biotin)

Binding Specificity: AA 188-199

Purpose: carbonic anhydrase XII (aa188-199), Biotinylated

Sequence: CSHLQHVKYK GQE.

Specificity: This antibody is expected to recognize all reported isoforms (NP_001209.1, NP_996808.1, NP_001280571.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in Human kidney lysates (calculated MW of 39.5 kDa according to NP_001209.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CA12

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Alternative Name: CA12

Background: CA12, carbonic anhydrase 12, CA-XII, CAXII, HsT18816, T18816, carbonate dehydratase XII, carbonic anhydrase XII, carbonic dehydratase, tumor antigen HOM-RCC-3.1.3

Gene ID: 771

NCBI Accession: NP_001209, NP_996808, NP_001280571