DYRK1A antibody (N-Term)

Catalog No. ABIN5539713

Product Details anti-DYRK1A Antibody

Target: DYRK1A (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 1A (DYRK1A))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DYRK1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: DYRK1A

Sequence: PHSHQYSDRR QPN

Specificity: This antibody is expected to recognize all reported isoforms (NP_001387.2, NP_569120.1, NP_567824.1, NP_569122.1). This antibody is not expected to cross-react with DYRK1B.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in lysates of cell line K562 (calculated MW of 85.6 kDa according to NP_001387.2). Recommended concentration: 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunoprecipitation: This product has been successfully used in IP on Human: Widowati et al, BMC Res Notes. 2018 May 15,11(1):297. PMID: 29764512.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-DYRK1A antibody (ABIN5539713)

Widowati, Bamberg-Lemper, Becker: "Mutational analysis of two residues in the DYRK homology box of the protein kinase DYRK1A." in: BMC research notes, Vol. 11, Issue 1, pp. 297, (2018) (PubMed).

Widowati, Ernst, Hausmann, Müller-Newen, Becker: "Functional characterization of DYRK1A missense variants associated with a syndromic form of intellectual deficiency and autism." in: Biology open, Vol. 7, Issue 4, (2018) (PubMed).

Target Details for DYRK1A

Target: DYRK1A (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 1A (DYRK1A))

Alternative Name: DYRK1A (DYRK1A Products)

Synonyms: DYRK1A antibody, dyrk antibody, dyrk1 antibody, hp86 antibody, mnb antibody, mnbh antibody, DYRK antibody, DYRK1 antibody, HP86 antibody, MNB antibody, MNBH antibody, MRD7 antibody, 2310043O08Rik antibody, D16Ertd272e antibody, D16Ertd493e antibody, Dyrk antibody, ENSMUSG00000074897 antibody, Gm10783 antibody, Mnbh antibody, Mp86 antibody, mmb antibody, PSK47 antibody, dual specificity tyrosine phosphorylation regulated kinase 1A antibody, dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A antibody, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a antibody, dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A S homeolog antibody, DYRK1A antibody, dyrk1a antibody, Dyrk1a antibody, dyrk1a.S antibody

Background: DYRK1A, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, DYRK, DYRK1, HP86, MNB, MNBH, MRD7, MNB/DYRK protein kinase, OTTHUMP00000109090, OTTHUMP00000109091, OTTHUMP00000109094, OTTHUMP00000174799, dual specificity YAK1-related kinase, d

Gene ID: 1859, 13548, 25255

NCBI Accession: NP_001387, NP_569120, NP_567824, NP_569122

Pathways: Mitotic G1-G1/S Phases