SIAH1 antibody (N-Term) (Biotin)

Catalog No. ABIN5539718

This Goat Polyclonal antibody specifically detects SIAH1 in WB, ELISA and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SIAH1 antibody (N-Term) (Biotin) (ABIN5539718)

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SIAH1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-SIAH1 Antibody (Biotin)

Binding Specificity: N-Term

Purpose: SIAH1, Biotinylated

Sequence: SRQTATALPT GTSKC.

Specificity: This antibody is expected to recognize both reported isoforms (NP_003022.3, NP_001006611.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 28 kDa band observed in Mouse Brain lysates (calculated MW of 31.1 kDa according to Mouse NP_033198.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SIAH1

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Alternative Name: SIAH1

Background: SIAH1, siah E3 ubiquitin protein ligase 1, SIAH1A, seven in absentia homolog 1, siah-1a

Gene ID: 6477, 20437, 140941

NCBI Accession: NP_003022, NP_001006611