FUBP1 antibody (AA 160-174)

Catalog No. ABIN5539722

The Goat Polyclonal anti-FUBP1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect FUBP1 in samples from Human.

Quick Overview for FUBP1 antibody (AA 160-174) (ABIN5539722)

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FUBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-FUBP1 Antibody

Binding Specificity: AA 160-174

Purpose: Fubp1 (mouse, aa160-174)

Sequence: DQIVEKGRPA PGFHH

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Tonsil shows staining in a selection of cells mostly outside the germinal centre. Recommended concentration: 4-8 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell line Jurkat (calculated MW of 67.4 kDa according to NP_476513.2). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FUBP1

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Alternative Name: Fubp1

Background: Fubp1, far upstream element (FUSE) binding protein 1, 9530027K12Rik, D3Ertd330e, FBP, Fubp, Fubp4, FUSE-binding protein 1, far upstream element (FUSE) binding protein 4, far upstream element-binding protein 1

Gene ID: 8880, 51886, 654496

NCBI Accession: NP_476513