GAPDH antibody (Internal Region) (Biotin)

Catalog No. ABIN5539725

The Goat Polyclonal anti-GAPDH antibody has been validated for WB, ELISA and IHC. It is suitable to detect GAPDH in samples from Human, Mouse and Rat.

Quick Overview for GAPDH antibody (Internal Region) (Biotin) (ABIN5539725)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This GAPDH antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GAPDH Antibody (Biotin)

Binding Specificity: Internal Region

Purpose: GAPDH (Internal), Biotinylated

Sequence: GVNHEKYDNS LK.

Specificity: This antibody is expected to recognize both reported isoforms (NP_002037.2, NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 36 kDa band observed in Human Liver lysates (calculated MW of 36.1 kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HEL-S-162eP, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH

Gene ID: 2597, 14433, 24383

NCBI Accession: NP_002037, NP_001243728