Galectin 3 antibody (Internal Region)

Catalog No. ABIN5539733

This Goat Polyclonal antibody specifically detects Galectin 3 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for Galectin 3 antibody (Internal Region) (ABIN5539733)

Target: Galectin 3 (LGALS3)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Galectin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Galectin 3 Antibody

Binding Specificity: Internal Region

Purpose: galectin-3 (mouse)

Sequence: NANRIVLDFR RGND

Specificity: Reported variants represent identical protein: NP_001139425.1, NP_034835.1

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 10 μg/mL.

Western Blot: Approx 26 kDa band observed in Mouse Heart lysates (calculated MW of 27.4 kDa according to NP_034835.1). Recommended concentration: 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Galectin 3

Target: Galectin 3 (LGALS3)

Alternative Name: Lgals3

Target Type: Chemical

Background: Lgals3, lectin, galactose binding, soluble 3, GBP, L-34, Mac-2, gal3, 35 kDa lectin, CBP 35, L-34 galactoside-binding lectin, OTTMUSP00000043688, OTTMUSP00000043689, OTTMUSP00000043690, carbohydrate-binding protein 35, gal-3, galactose-specific lectin 3,

Gene ID: 16854

NCBI Accession: NP_034835

Pathways: RTK Signaling