Myosin 9 antibody (Internal Region)

Catalog No. ABIN5539742

This Goat Polyclonal antibody specifically detects Myosin 9 in WB and ELISA. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for Myosin 9 antibody (Internal Region) (ABIN5539742)

Target: Myosin 9 (MYH9)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Myosin 9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Myosin 9 Antibody

Binding Specificity: Internal Region

Purpose: MYH9

Sequence: DQINTDLNLE RSH

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 250 kDa band observed in lysates of Human Peripheral Blood Mononucleocytes and of NIH3T3 (calculated MW of 227 kDa according to Human NP_002464.1 and to Mouse NP_071855.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Myosin 9 antibody (ABIN5539742)

Harms, Borengasser, Kumar, Sarvetnick: "Anti-human Interleukin(IL)-4 Clone 8D4-8 Cross-Reacts With Myosin-9 Associated With Apoptotic Cells and Should Not Be Used for Flow Cytometry Applications Querying IL-4 Expression." in: Frontiers in cell and developmental biology, Vol. 7, pp. 46, (2019) (PubMed).

Target Details for Myosin 9

Target: Myosin 9 (MYH9)

Alternative Name: MYH9

Background: Myosin, heavy chain 9, non-muscle, RP1-68O2.1, DFNA17, EPSTS, FTNS, MGC104539, MHA, NMHC-II-A, NMMHCA, MYH9 variant protein, OTTHUMP00000028706, cellular myosin heavy chain, type A, myosin, heavy polypeptide 9, non-muscle, non-muscle myosin heavy chain, n

Gene ID: 4627

NCBI Accession: NP_002464

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Integrin Complex