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UBE2R2 antibody (N-Term)

This Goat Polyclonal antibody specifically detects UBE2R2 in ELISA, IHC and IEM. It exhibits reactivity toward Human and Rat.
Catalog No. ABIN5539763

Quick Overview for UBE2R2 antibody (N-Term) (ABIN5539763)

Target

See all UBE2R2 Antibodies
UBE2R2 (Ubiquitin-Conjugating Enzyme E2R 2 (UBE2R2))

Reactivity

  • 29
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Rat

Host

  • 27
  • 2
Goat

Clonality

  • 27
  • 2
Polyclonal

Conjugate

  • 10
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This UBE2R2 antibody is un-conjugated

Application

  • 29
  • 13
  • 13
  • 12
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC), Immunoelectron Microscopy (IEM)

Grade

Verified
  • Binding Specificity

    • 15
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term

    Purpose

    UBC3B

    Sequence

    AQQQMTSSQK ALM

    Cross-Reactivity

    Human, Mouse, Rat

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Testis shows textured nuclear staining of the primary spermatocytes . Recommended concentration, 2-4 μg/mL.

    Western Blot: No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1 μg/mL. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

    Peptide ELISA: antibody detection limit dilution 1:4000.

    Comment

    Immunoelectron microscopy: This product stained chromatoid bodies in Rat spermatids.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    UBE2R2 (Ubiquitin-Conjugating Enzyme E2R 2 (UBE2R2))

    Alternative Name

    UBE2R2

    Background

    UBE2R2, UBC3B, CDC34B, FLJ20419, MGC10481, ubiquitin-conjugating enzyme E2R 2, ubiquitin-conjugating enzyme UBC3B

    Gene ID

    54926, 67615

    NCBI Accession

    NP_060281
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