UBE2R2 antibody (N-Term)

Catalog No. ABIN5539763

This Goat Polyclonal antibody specifically detects UBE2R2 in ELISA, IHC and IEM. It exhibits reactivity toward Human and Rat.

Quick Overview for UBE2R2 antibody (N-Term) (ABIN5539763)

Target: UBE2R2 (Ubiquitin-Conjugating Enzyme E2R 2 (UBE2R2))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2R2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunoelectron Microscopy (IEM)

Grade: Verified

Product Details anti-UBE2R2 Antibody

Binding Specificity: N-Term

Purpose: UBC3B

Sequence: AQQQMTSSQK ALM

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows textured nuclear staining of the primary spermatocytes . Recommended concentration, 2-4 μg/mL.

Western Blot: No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1 μg/mL. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunoelectron microscopy: This product stained chromatoid bodies in Rat spermatids.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBE2R2

Target: UBE2R2 (Ubiquitin-Conjugating Enzyme E2R 2 (UBE2R2))

Alternative Name: UBE2R2

Background: UBE2R2, UBC3B, CDC34B, FLJ20419, MGC10481, ubiquitin-conjugating enzyme E2R 2, ubiquitin-conjugating enzyme UBC3B

Gene ID: 54926, 67615

NCBI Accession: NP_060281