BMI1 antibody (AA 237-251)

Catalog No. ABIN5539767

The Goat Polyclonal anti-BMI1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect BMI1 in samples from Human, Mouse and Pig.

Quick Overview for BMI1 antibody (AA 237-251) (ABIN5539767)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-BMI1 Antibody

Binding Specificity: AA 237-251

Purpose: BMI1 (aa237-251)

Sequence: SHQRDGLTNA GELES

Cross-Reactivity: Dog, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 40 kDa band observed in nuclear lysates of cell lines K562 and NIH3T3 (calculated MW of 36.9 kDa according to NP_005171.4). This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1

Background: BMI1, BMI1 polycomb ring finger oncogene, FLVI2/BMI1, PCGF4, RNF51, B lymphoma Mo-MLV insertion region 1 homolog, flvi-2/bmi-1, murine leukemia viral (bmi-1) oncogene homolog, polycomb complex protein BMI-1, polycomb group RING finger protein 4, polycomb

Gene ID: 648, 12151

NCBI Accession: NP_005171

Pathways: Cell Division Cycle, Autophagy