GLP1R antibody (Internal Region)

Catalog No. ABIN5539774

This anti-GLP1R antibody is a Goat Polyclonal antibody detecting GLP1R in WB, ELISA, IF and FACS. Suitable for Human, Rat and Pig.

Quick Overview for GLP1R antibody (Internal Region) (ABIN5539774)

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Reactivity: Human, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This GLP1R antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-GLP1R Antibody

Binding Specificity: Internal Region

Purpose: GLP1R

Sequence: QHQWDGLLSY QD

Specificity: The immunizing peptide represents part of an extracellular region.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Hippocampus and in Rat and Pig Brain lysates (calculated MW of 53.0 kDa according to Human NP_002053.3, 52.9 kDa according to Rat NP_036860.1 and 52.7 kDa according to Pig NP_001243523.1). Recommended concen

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GLP1R

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Alternative Name: GLP1R

Background: Glp1r, glucagon-like peptide 1 receptor, GLP-1R, GLP1Rc, GLP-1 receptor, GLP-1-R

Gene ID: 2740, 14652, 25051

NCBI Accession: NP_002053

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, cAMP Metabolic Process, Feeding Behaviour