GLP1R antibody (2nd Extracellular Loop) (FITC)

Catalog No. ABIN7043220

The FITC-conjugated Rabbit Polyclonal anti-GLP1R antibody (ABIN7043220) specifically detects GLP1R in FACS and LCI. The antibody is reactive with Rat samples.

Quick Overview for GLP1R antibody (2nd Extracellular Loop) (FITC) (ABIN7043220)

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLP1R antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GLP1R Antibody (FITC)

Binding Specificity: 2nd Extracellular Loop, AA 288-301

Purpose: A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor Conjugated to the Fluorescent Dye FITC

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,human - 13,14 amino acid residues identical

Characteristics: Anti-GLP1R (extracellular) Antibody (ABIN7043219, ABIN7044406 and ABIN7044407) is a highly specific antibody directed against an epitope of the rat Glucagon-like peptide 1 receptor. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize GLP1R from human, rat, and mouse samples. \nAnti-GLP1R (extracellular)-FITC Antibody (ABIN7043219, ABIN7044406 and ABIN7044407)-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R

Isotype: IgG

Application Details

Application Notes:

WB: The optimal concentration should be determined by the user

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235607)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GLP1R

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Alternative Name: GLP1R

Background:

Synonyms: Glucagon-like peptide 1 receptor, GLP-1 receptor

Description: Glucagon-like peptide 1 (GLP-1) is a hormone secreted by the intestine following a meal1. This hormone is known to enhance the secretion of insulin, important to regulate glucose blood levels2,3. In pancreatic b-cells, other than stimulating insulin secretion, GLP-1 action is also important for b-cell mass expansion2.GLP-1 exerts its biological functions through Glucagon-like peptide 1 receptor (GLP-1 receptor), a member of the G-protein coupled receptor superfamily. Like all members this receptor spans the membrane seven times. GLP-1 receptor couples with Gs, thereby stimulates adenylate cyclase which consequently leads to an increase in cAMP2.The receptor is expressed in various tissues, namely the pancreas, adipose tissue, muscle, heart, gastrointestinal tract and liver. GLP-1 receptor is also found in the central nervous system where it provides a neuroprotective effect4.The glucagon-like peptide 1 system may serve as a therapeutic treatment in diabetes mellitus, in order to maintain b-cell mass expansion2. In addition, the receptor may serve as a therapy target in various neurodegenerative diseases like Alzheimer's and Parkinson's disease4.

Gene ID: 25051

NCBI Accession: NM_002062

UniProt: P32301

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, cAMP Metabolic Process, Feeding Behaviour