GLRA1 antibody (Internal Region)

Catalog No. ABIN5539834

This anti-GLRA1 antibody is a Goat Polyclonal antibody detecting GLRA1 in WB, ELISA and IHC. Suitable for Human, Rat and Mouse.

Quick Overview for GLRA1 antibody (Internal Region) (ABIN5539834)

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GLRA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GLRA1 Antibody

Binding Specificity: Internal Region

Purpose: Glycine receptor, alpha 1 (intracellular)

Sequence: CLQAKDGISV KGAN

Specificity: The immunizing peptide represents part of the cytoplasmic domain. The antibody is not expected to cross-react with other glycine receptor alpha proteins.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Lung and Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Mouse and Rat Testis lysates (calculated MW of 51.7 kDa according to Mouse NP_065238.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL. Primary incubat

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GLRA1

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Alternative Name: Glra1

Background: Glra1, glycine receptor, alpha 1, GLYRA1, glycine receptor 48 kDa subunit, glycine receptor strychnine-binding subunit, glycine receptor subunit alpha-1, glycine receptor, alpha 1 subunit

Gene ID: 2741, 14654, 25674

NCBI Accession: NP_037265

Pathways: Synaptic Membrane