MOB2 antibody (AA 128-140)

Catalog No. ABIN5539861

This anti-MOB2 antibody is a Goat Polyclonal antibody detecting MOB2 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for MOB2 antibody (AA 128-140) (ABIN5539861)

Target: MOB2 (MOB Kinase Activator 2 (MOB2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MOB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MOB2 Antibody

Binding Specificity: AA 128-140

Purpose: MOB2 (aa128-140)

Sequence: DERGKKVKCT APQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_001165694.1, NP_443731.2) only.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Lung shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx 30 kDa band observed in lysates of cell line Jurkat (calculated MW of 30.2 kDa according to NP_001165694.1). Recommended concentration: 0.02-0.06 μg/mL. An additional band of unknown identity was also consistently observed at 48 kDa. Thi

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MOB2

Target: MOB2 (MOB Kinase Activator 2 (MOB2))

Alternative Name: MOB2

Background: MOB2, HCCA2, HCCA2 protein, mob2 homolog, mps one binder kinase activator-like 2

Gene ID: 81532, 101513, 499288

NCBI Accession: NP_001165694, NP_443731