MDH2 antibody (Internal Region)

Catalog No. ABIN5539864

The Goat Polyclonal anti-MDH2 antibody (ABIN5539864) specifically detects MDH2 in WB, IHC and ELISA. The antibody is reactive with Human, Mouse, Rat and Pig samples.

Quick Overview for MDH2 antibody (Internal Region) (ABIN5539864)

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This MDH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-MDH2 Antibody

Binding Specificity: Internal Region

Purpose: MDH2

Sequence: TRDDLFNTNA T

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in lysates of cell lines HeLa, HepG2 and NIH3T3 (calculated MW of 35.5 kDa according to Human NP_005909.2 and 35.6 kDa according to Mouse NP_032643.2 ). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation wa

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MDH2

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Alternative Name: MDH2

Background: MDH2, malate dehydrogenase 2, NAD (mitochondrial), M-MDH, MDH, MGC:3559, MOR1, malate dehydrogenase, mitochondrial

Gene ID: 4191, 17448, 81829

NCBI Accession: NP_005909