MDH2 antibody (Internal Region)

Catalog No. ABIN5539864

This anti-MDH2 antibody is a Goat Polyclonal antibody detecting MDH2 in WB, ELISA and IHC. Suitable for Human, Mouse, Rat and Pig.

Quick Overview for MDH2 antibody (Internal Region) (ABIN5539864)

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This MDH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MDH2 Antibody

Binding Specificity: Internal Region

Purpose: MDH2

Sequence: TRDDLFNTNA T

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in lysates of cell lines HeLa, HepG2 and NIH3T3 (calculated MW of 35.5 kDa according to Human NP_005909.2 and 35.6 kDa according to Mouse NP_032643.2 ). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation wa

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MDH2

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Alternative Name: MDH2

Background: MDH2, malate dehydrogenase 2, NAD (mitochondrial), M-MDH, MDH, MGC:3559, MOR1, malate dehydrogenase, mitochondrial

Gene ID: 4191, 17448, 81829

NCBI Accession: NP_005909