ORC4 antibody (C-Term) (Biotin)

Catalog No. ABIN5539875

Product Details anti-ORC4 Antibody (Biotin)

Target: ORC4 (Origin Recognition Complex, Subunit 4 (ORC4))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ORC4 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Purpose: ORC4L, Biotinylated

Sequence: DVRQWATSSL SWL.

Specificity: This antibody is expected to recognize all reported isoforms (NP_002543.2, NP_001177810.1, NP_001177811.1). Reported variants represent identical protein: XP_011509557.1, NP_002543.2, NP_001177808.1, NP_859526.1, NP_859525.1 Reported variants represent id

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in Jurkat nuclear lysates (calculated MW of 50.4 kDa according to NP_002543.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Chromatin immunoprecipitation: reported by a customer to work very well in ChIP.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ORC4

Target: ORC4 (Origin Recognition Complex, Subunit 4 (ORC4))

Alternative Name: ORC4 (ORC4 Products)

Background: ORC4, origin recognition complex subunit 4, ORC4L, ORC4P, origin recognition complex, subunit 4 homolog

Gene ID: 5000, 26428, 295596

NCBI Accession: NP_002543, NP_001177810, NP_001177811

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA