RIF1 antibody (C-Term)

Catalog No. ABIN5539883

This Goat Polyclonal antibody specifically detects RIF1 in ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for RIF1 antibody (C-Term) (ABIN5539883)

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RIF1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-RIF1 Antibody

Binding Specificity: C-Term

Purpose: RIF1

Sequence: RNLQSRWRSP SHEN

Specificity: This antibody is expected to recognize both reported isoforms (NP_060621.3, NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3, HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RIF1

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Alternative Name: RIF1

Background: RIF1, RAP1 interacting factor homolog (yeast), DKFZp434D1026, DKFZp781N1478, FLJ12870, OTTHUMP00000162712, OTTHUMP00000207206, OTTHUMP00000207207, OTTHUMP00000207208, OTTHUMP00000207210, rap1-interacting factor 1 homolog, telomere-associated protein RIF1

Gene ID: 55183, 51869

NCBI Accession: NP_060621, NP_001171134

Pathways: Stem Cell Maintenance