YAP1 antibody (Internal Region)

Catalog No. ABIN5539893

The Goat Polyclonal anti-YAP1 antibody has been validated for WB and ELISA. It is suitable to detect YAP1 in samples from Dog.

Quick Overview for YAP1 antibody (Internal Region) (ABIN5539893)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reactivity: Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-YAP1 Antibody

Binding Specificity: Internal Region

Purpose: YAP1

Sequence: TYHSRDESTD S

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in lysates of cell line MDCK (calculated MW of 50.3 kDa according to Dog XP_536601.2). Data obtained from an anonymous customer. Recommended concentration: 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP1

Background: Yes-associated protein 1, 65 kDa, YAP, YAP2, YAP65, Yes-associated protein 1, 65 kD, Yes-associated protein 1, 65 kDa, yes-associated protein 2

Gene ID: 10413, 22601, 363014

NCBI Accession: NP_006097

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha