CHRNA7 antibody (Internal Region)

Catalog No. ABIN5539917

The Goat Polyclonal anti-CHRNA7 antibody has been validated for IHC and ELISA. It is suitable to detect CHRNA7 in samples from Human and Rat.

Quick Overview for CHRNA7 antibody (Internal Region) (ABIN5539917)

Target: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CHRNA7 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CHRNA7 Antibody

Binding Specificity: Internal Region

Purpose: CHRNA7

Sequence: KRPGEDKVRP ACQHKQ

Specificity: This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Rat Cerebral Cortex and Rat Hippocampus shows membranous staining in neural cells. Recommended concentration: 5-10 μg/mL. Paraffin embedded Human Brain (Cortex). Recommended concentration: 3-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHRNA7

Target: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Alternative Name: CHRNA7

Background: CHRNA7, cholinergic receptor, nicotinic, alpha 7, NACHRA7, a7 nicotinic acetylcholine receptor, alpha 7 neuronal nicotinic acetylcholine receptor, alpha-7 nicotinic cholinergic receptor subunit, alpha7 nicotinic receptor subunit, cholinergic receptor, nic

Gene ID: 1139, 11441, 25302

NCBI Accession: NP_000737

Pathways: Synaptic Membrane