CHRNA7 antibody (N-Term)

Catalog No. ABIN2792250

The Rabbit Polyclonal anti-CHRNA7 antibody (ABIN2792250) specifically detects CHRNA7 in WB. The antibody is reactive with Human, Rat, Mouse and Cow samples.

Quick Overview for CHRNA7 antibody (N-Term) (ABIN2792250)

Target: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Reactivity: Human, Rat, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNA7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHRNA7 Antibody

Binding Specificity: N-Term

Purpose: CHRNA7 Antibody - N-terminal region

Sequence: QGEFQRKLYK ELVKNYNPLE RPVANDSQPL TVYFSLSLLQ IMDVDEKNQV

Predicted Reactivity: Cow: 93%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CHRNA7. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHRNA7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHRNA7

Target: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Alternative Name: CHRNA7

Background:

Targeted Subunit: alpha-7

Background Information: The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. CHRNA7 is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from CHRNA7 and a novel FAM7A gene.

Gene Name: Cholinergic receptor, nicotinic, alpha 7 (neuronal)

Alternative Symbols: NACHRA7, CHRNA7-2

Protein Name: Neuronal acetylcholine receptor subunit alpha-7

Molecular Weight: 56 kDa

Gene ID: 1139

NCBI Accession: NP_000737

UniProt: P36544

Pathways: Synaptic Membrane