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CHRNA7 antibody (AA 441-502)

Name: CHRNA7 antibody (AA 441-502)
SKU: ABIN736911
Availability: InStock

The Rabbit Polyclonal anti-CHRNA7 antibody (ABIN736911) specifically detects CHRNA7 in WB, ELISA, ICC, IF (cc), IF (p), IHC (p) and IHC (fro). The antibody is reactive with Human, Rat and Mouse samples.

Mouse brain lysates probed with Rabbit Anti-CHRNA7 Polyclonal Antibody, Unconjugated at 1:500 for 90 min at 37˚C. (CHRNA7 antibody (AA 441-502))

Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-CHRNA7 Polyclonal Antibody, Unconjugated (ABIN736911) at 1:200, followed by conjugation to the secondary antibody and DAB staining (CHRNA7 antibody (AA 441-502))

IHC

Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-CHRNA7 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (CHRNA7 antibody (AA 441-502))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CHRNA7 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated-Cy3)used at 1:200 dilution for 40 minutes at 37°C. (CHRNA7 antibody (AA 441-502))

IF (p)

HEK 293T cells were stained with CHRNA7 Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at room temperature, followed by secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated -Cy3) used at 1:400 dilution for 90 minutes at 37°C. (CHRNA7 antibody (AA 441-502))

IF (cc)

5 images

Catalog No. ABIN736911

$384.62

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 4 to 7 Business Days

Quick Overview for CHRNA7 antibody (AA 441-502) (ABIN736911)

Target

See all CHRNA7 Antibodies

CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Reactivity

Human, Rat, Mouse

Host

Rabbit

Clonality

Polyclonal

Conjugate

This CHRNA7 antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product CHRNA7 Primary Antibody

Binding Specificity
14

8

6

4

4

3

2

2

2

1

1

1

1

1

1

1
AA 441-502

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Chicken

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CHRNA7

Isotype

IgG
anti-Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7) (AA 52-259) antibody
CHRNA7 Reactivity: Human IHC, ELISA, FACS Host: Mouse Monoclonal 7F10G1 unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7) (AA 52-259) antibody
CHRNA7 Reactivity: Human, Rat WB, ELISA, FACS Host: Mouse Monoclonal 7F10F1 unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7) (AA 23-230) antibody
CHRNA7 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7) (AA 23-230) antibody
CHRNA7 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7) (N-Term) antibody
CHRNA7 Reactivity: Human, Rat, Mouse, Cow WB Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7) (AA 31-42), (Extracellular) antibody
KO Validated CHRNA7 Reactivity: Rat WB, IHC, FACS, LCI, IF, IC Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7) (AA 31-42), (Extracellular) antibody
CHRNA7 Reactivity: Rat WB, IHC Host: Guinea Pig Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7) (Internal Region) antibody
Verified CHRNA7 Reactivity: Human, Rat IHC, ELISA Host: Goat Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7) (C-Term) antibody
CHRNA7 Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Guinea Pig, Rabbit WB Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7) (AA 31-42), (Extracellular) antibody (FITC)
CHRNA7 Reactivity: Rat FACS, LCI Host: Rabbit Polyclonal FITC

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Alternative Name

CHRNA7

Background

Synonyms: NACHRA7, CHRNA7-2, Neuronal acetylcholine receptor subunit alpha-7, CHRNA7
Background: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.

Gene ID

1139

UniProt

P36544

Pathways

Synaptic Membrane