Complement Factor H antibody

Catalog No. ABIN5540274

The Mouse Monoclonal anti-Complement Factor H antibody is suitable to detect Complement Factor H in samples from Rat and Mouse. It has been validated for WB, EIA and IHC (fro).

Quick Overview for Complement Factor H antibody (ABIN5540274)

Target: Complement Factor H (CFH)

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement Factor H antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1A2

Product Details anti-Complement Factor H Antibody

Specificity: The monoclonal antibody 1A2 recognizes Mouse complement factor H (CFH).

Purification: Purified

Immunogen: Mouse factor H-human IgG fusion protein.

Isotype: IgG1

Application Details

Application Notes: Immuno Assays. Western blot . Immunohistochemistry on Frozen Sections The typical starting working dilution is 1/50.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.02 % Sodium Azide Stabilizers: 0.1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Background: CFH is the first regulatory protein of the alternative pathway of the complement system. There are three pathways of complement activation classical, alternative, and lectin activation pathway. These pathways converge to form C3/C5 convertases that generate C3a, C3b,C5a, and C5b, each with substantial biologic activity. Complement regulators are necessary to prevent the injudicious production of these mediators and potential injury to self-tissue. The plasma proteins CFH, C4-binding protein and the cell membrane proteins complement receptor 1 (CR1 CD35), decay-accelerating factor (CD55), and membrane co-factor protein (CD46) all are members of the regulators of complement activation family. These proteins have natural affinity for C3b and/or C4b, which confers on them the ability to accelerate the intrinsic decay of C3/C5 convertases and/or act as co-factor for the cleavage and inactivation (i) of C3b and C4b by complement factor I (CFI). Genetic human analyses reveals a clear association of CFH with different human diseases. These include diseases of the kidney, the atypical form of Hemolytic Uremic Syndrome (aHUS) and membranoproliferative glomerulonephritis (MPGN). Furthermore, CFH is associated with age-related macular degeneration (AMD), a disease of the eye.

UniProt: P06909

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin