EPAS1 antibody (N-Term)

Catalog No. ABIN5540327

This Rabbit Polyclonal antibody specifically detects EPAS1 in WB, ICC, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Rat and Mouse.

Quick Overview for EPAS1 antibody (N-Term) (ABIN5540327)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-EPAS1 Antibody

Binding Specificity: AA 202-240, N-Term

Sequence: YNNCPPHSSL CGYKEPLLSC LIIMCEPIQH PSHMDIPLD

Specificity: This antibody detects HIF2A / HIF2 alpha (202-240). No cross reactivity with other proteins.

Purification: Immunogen Affinity Chromatography

Immunogen: A synthetic peptide corresponding to a sequence at N-terminus of Rat HIF-2-alpha (202-240)

Isotype: IgG

Application Details

Application Notes: Western blot: 0.1-0.5 μg/mL with the appropriate system to detect HIF-2α in cells and tissues. Immunohistochemistry on Paraffin Sections: 0.5-1 μg/mL to detect HIF-2α in formalin fixed and paraffin embedded tissues. Bioling the sections is required. Immunohistochemistry on Frozen Sections: 0.5-1 μg/mL to detect HIF-2α in formalin or acetone fixed tissues.

Restrictions: For Research Use only

Handling

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg Sidium Azide

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: hif2a,hif2 alpha

Background: HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88 % identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event. Moreover, the predicted protein shares 48 % sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration.

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect