GFRA3 antibody

Catalog No. ABIN5540354

This Chicken Polyclonal antibody specifically detects GFRA3 in WB, IF, EIA and ICC. It exhibits reactivity toward Human.

Quick Overview for GFRA3 antibody (ABIN5540354)

Target: GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFRA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC)

Product Details anti-GFRA3 Antibody

Specificity: This antibody detects Human GFRa-3 and doesn't cross-react with hGFRa-1, hGFRa-2, hGFRa-4. Other species not tested.

Purification: Affinity Chromatography

Immunogen: Recombinant His-tagged human GFRa-3 protein produced using CHO-based. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.

Isotype: IgY

Application Details

Application Notes: ELISA: 1/160000-1/320000. Western blot: 1/5000-1/10000. Immunofluorescence: 1/500-1/3000.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Concentrated Ammonium Sulphate in PBS pH 7.4

Storage: 4 °C

Storage Comment: Upon receipt, store at 2-8°C. As product is (NH4) 2 SO 4 precipitate, mix well by pipetting or vortexing prior use. Shelf life: One year from despatch.

Target Details for GFRA3

Target: GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))

Alternative Name: gfra3,gdnfr-alpha 3

Background: Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFRa-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).

UniProt: O60609