GFRA3 antibody (Extracellular, N-Term)

Catalog No. ABIN7886746

This Rabbit Polyclonal antibody specifically detects GFRA3 in WB. It exhibits reactivity toward Mouse.

Quick Overview for GFRA3 antibody (Extracellular, N-Term) (ABIN7886746)

Target: GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFRA3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GFRA3 Antibody

Binding Specificity: AA 172-185, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to GDNF Family Receptor α3

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse,rat,human - 13,14 amino acid residues identical

Characteristics: Anti-GFR alpha 3 (extracellular) Antibody (ABIN7043212, ABIN7044779 and ABIN7044780) is a highly specific antibody directed against an epitope of mouse GDNF receptor α3. The antibody can be used in western blot analysis. It has been designed to recognize GFR α3 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CDRLRKAYGEA(S)SG, corresponding to amino acid residues 172-185 of mouse GFRA3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235580)

Blocking Peptide: (ABIN7235580)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GFRA3

Target: GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))

Alternative Name: GFRA3

Background:

Synonyms: GFRA3, GFRα3, GDNF family receptor α3, GDNF receptor alpha 3

Description: GFRα3 (GDNF Family Receptor α3) belongs to the glial cell line derived neurotrophic factor (GDNF) family of receptors which contains four members GFRα1-4. The GDNF family of ligands (GFLs)- GDNF, Neurturin, Artemin, and Persephin bind to co-receptors GFRα1-4 and activate Ret receptor tyrosine kinase. GDNF-family receptor α is glycosyl-phosphatidylinositol anchored to the cell surface. GFRα3 structure contains three homologous domains (D1, D2 and D3) and a C-terminal extension1-4.GFRα3 is a highly selective receptor for Artemin (Artn). Artemin is a vasculature-derived neurotrophic factor that modulates the development and function of sensory neurons and participates in the pathophysiology of peripheral inflammation and pain hyperalgesia.In adults, GFRα3 is restricted to neurons of the peripheral nervous system. The protein is expressed by a subpopulation of nociceptive sensory neurons which also express the Ret receptor tyrosine kinase, TRPV1, TRPA11,2,3.

Gene ID: 14587

NCBI Accession: NM_001496

UniProt: O35118