IL-7 antibody

Catalog No. ABIN5540412

The Rabbit Polyclonal anti-IL-7 antibody has been validated for WB, Neut and EIA. It is suitable to detect IL-7 in samples from Rat.

Quick Overview for IL-7 antibody (ABIN5540412)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-7 antibody is un-conjugated

Application: Western Blotting (WB), Neutralization (Neut), Enzyme Immunoassay (EIA)

Product Details anti-IL-7 Antibody

Specificity: This antibody recognizes Rat IL7. Other species not tested.

Purification: Affinity Chromatography employing immobilized Human IL7 matrix

Immunogen: Highly pure (> 98%) E.coli derived recombinant Rat IL7 (Cat.-No PA266)

Application Details

Application Notes: Sandwich ELISA: To detect Rat IL-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-7 (Cat.-No AP32852BT-N or AP32852BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-7. Western Blot: To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND 50 ] of the biological activity of Rat IL-7 (5.0 ng/mL), a concentration of 0.14-0.21 μg/mL of this antibody is required.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: interleukin-7,il7

Background: IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive.

Pathways: JAK-STAT Signaling