Syndecan 2 antibody

Catalog No. ABIN5540684

This Mouse Monoclonal antibody specifically detects Syndecan 2 in WB, EIA and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Syndecan 2 antibody (ABIN5540684)

Target: Syndecan 2 (SDC2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Syndecan 2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2E2-B5

Product Details anti-Syndecan 2 Antibody

Specificity: This Monoclonal antibody is specific for a core protein epitope of a Human small basement membrane Heparan Sulfate Proteoglycan (HS-PG). AM20231PU recognizes an epitope different from that recognized by clones 1F10/B8 (Cat.No AM02102PU) and 2H7/G11 (Cat.-No AM02103PU). Species: Human.

Purification: Protein G Chromatography

Immunogen: Purified Human small basement membrane Heparan Sulfate Proteoglycan (HS-PG)

Isotype: IgG1

Application Details

Application Notes: ELISA (less than 1 μg/mL). Western blot (1 μg/mL). Immunohistochemistry (1 μg/mL)

Restrictions: For Research Use only

Handling

Buffer: 50 mM TRIS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Syndecan 2

Target: Syndecan 2 (SDC2)

Alternative Name: hspg1,syndecan-2

Background: The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohistoche-mistry to the basement membrane. Amino acid sequence ana-lysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.

UniProt: P34741

Pathways: Glycosaminoglycan Metabolic Process