PKM2 antibody (AA 1-531)

Catalog No. ABIN5540840

This Mouse Monoclonal antibody specifically detects PKM2 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for PKM2 antibody (AA 1-531) (ABIN5540840)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: AT1B10

Product Details anti-PKM2 Antibody

Binding Specificity: AA 1-531

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human PKM2 (1-531aa) purified from E.coli.

Isotype: IgG1

Application Details

Application Notes: The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000 and Immunofluorescence is 1:250.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: pkm2

Background: PKM2 (pyruvate kinase M2), its association with phospho-tyrosine (pTyr) motifs, enables cells to use predominantly aerobic glycolysis instead of oxidative phosphorylation. Also PKM2 suggest that this change benefits cancer cells by enabling them to redirect glycolytic metabolites away from oxidation and energy production and, instead, toward anabolic processes and biosynthesis. PKM2-mediated generation of the aerobic glycolytic phenotype, although wasteful in terms of glucose consumption and waste generation, could provide a variety of advantages for cancerous cells. In addition nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death.

UniProt: P14618

Pathways: Warburg Effect