CBX1 antibody

Catalog No. ABIN5540983

This anti-CBX1 antibody is a Mouse Monoclonal antibody detecting CBX1 in IHC (p). Suitable for Human, Mouse and Pig.

Quick Overview for CBX1 antibody (ABIN5540983)

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Reactivity: Human, Mouse, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CBX1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1MOD-1A9

Product Details anti-CBX1 Antibody

Specificity: This antibody detects HP1β.

Purification: Purified

Immunogen: Recombinant mouse HP1beta

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 4 μg/mL (1:50). Microwave pretreatment in citrate buffer is recommended for antigen retrieval. Suggested Positive control: Human / Swine brain cortex.

Restrictions: For Research Use only

Handling

Buffer: Buffer System: Phosphate buffered saline pH 7.2 (PBS) Preservatives: 0.01 % Kathon Stabilizers: 5 mg/mL bovine serum albumin (BSA)

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CBX1

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Alternative Name: cbx1

Background: Heterochromatin Protein 1 (HP1) (Chromobox Homolog, CBX) includes three isoforms in mammals, designated α, β, and γ. HP1 proteins are approximately 25 kDa in size. They are highly conserved adapter proteins with important regulatory functions in the cell nucleus. These functions include notably gene silencing by heterochromatin formation. HP1 proteins are fundamental units of heterochromatin packaging that are enriched at the centromeres and telomeres of nearly all Eukaryotic chromosomes. HP1 β and γ also localize to euchromatic sites in the genome. Members of the HP1 family are characterized by an N-terminal chromodomain and a C- terminal chromoshadow domain, separated by a Hinge region. Chromodomain and chromoshadow domain establish secondary interactions with a large number of other proteins. HP1 proteins are subject to multiple types of post - translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation.

UniProt: P83916