ALDOA antibody (Biotin)

Catalog No. ABIN5541022

The Biotin-conjugated Goat Polyclonal anti-ALDOA antibody (ABIN5541022) specifically detects ALDOA in WB, IP and EIA. The antibody is reactive with Human and Rabbit samples.

Quick Overview for ALDOA antibody (Biotin) (ABIN5541022)

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivity: Human, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This ALDOA antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-ALDOA Antibody (Biotin)

Specificity: This antibody detects rabbit aldolase. Cross reactivity against aldolase from other tissues and species may also occur. Immunoelectrophoresis gives a single precipitin arc against anti-goat serum, purified and partially purified aldolase [rabbit muscle]. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines. Species: Rabbit, Human.

Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Aldolase from rabbit muscle

Application Details

Application Notes: Western blot :1/500-1/5,000. Immunoprecipitation : 1/100. Use approximately 5 μL of antibody to immunoprecipitate 50 μL of protein lysate. ELISA : 1/5,000-1/20,000. This antibody has been assayed against 1.0 μg of aldolase [rabbit muscle] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested for this product.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Alternative Name: aldolase a,aldoa

UniProt: P00883

Pathways: Ribonucleoside Biosynthetic Process