ASF1B antibody (AA 1-202)

Catalog No. ABIN5541030

The Mouse Monoclonal anti-ASF1B antibody has been validated for WB and EIA. It is suitable to detect ASF1B in samples from Human.

Quick Overview for ASF1B antibody (AA 1-202) (ABIN5541030)

Target: ASF1B (ASF1 Anti-Silencing Function 1 Homolog B (ASF1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ASF1B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: AT1D4

Product Details anti-ASF1B Antibody

Binding Specificity: AA 1-202

Specificity: Recognizes Human ASF1B. Other species not tested.

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human ASF1B (1-202aa) purified from E. coli.

Isotype: IgG2b

Application Details

Application Notes: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ASF1B

Target: ASF1B (ASF1 Anti-Silencing Function 1 Homolog B (ASF1B))

Alternative Name: asf1b

Background: ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. Delivering histone H3/H4 dimers to CAF-1 or HIRA histone deposition complexes to facilitate replication-coupled and replication-independent nucleosome assembly on DNA. Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to replicative stress.

UniProt: Q9NVP2

Pathways: Regulation of Cell Size